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參數(shù)資料
| 型號(hào)： | S7804 |
| 廠商： | Electronic Theatre Controls, Inc. |
| 英文描述： | CpG WIZ Prader-Willi/Angelman Amplification Kit |
| 中文描述： | 中央人民政府武進(jìn)Prader-Willi/Angelman擴(kuò)增試劑盒 |
| 文件頁(yè)數(shù)： | 6/32頁(yè) |
| 文件大?。?/td> | 386K |
| 代理商： | S7804 |
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2
Principles of the Technique
MSP, performed using the CpGenome DNA Modification Kit and CpG
WIZ Amplification Kits, permits sensitive detection of altered DNA. Due to
the fact that it is a PCR-based assay, it is extremely sensitive, facilitating the
detection of low numbers of methylated alleles and the study of samples
containing low amounts of DNA. MSP also allows examination of all CpG sites,
not just those within sequences recognized by methylation sensitive restriction
enzymes. Increasing the number of such sites which can be assessed allows
rapid, fine mapping of methylation patterns throughout CpG regions. In
addition, the bisulfite modification is ideally suited for analysis of CpG islands
since it converts the majority of cytosines to uracils, making a region of the
genome which is CG-rich less difficult to amplify by PCR.
Methylation-specific PCR employs an initial bisulfite reaction to modify the
DNA, followed by a "hot start" PCR amplification with specific primers
designed to distinguish methylated DNA from unmethylated DNA. As shown in
Figure 1, in the bisulfite reaction, all unmethylated cytosines are converted to
uracils while 5-methylcytosines remain unaltered. Thus, the sequence of the
treated DNA will differ if the DNA is originally methylated vs. unmethylated.
Primers contained in CpG WIZ
Amplification Kits are designed to specifically
amplify each of the sequences based upon these chemically-induced differences.
If the sample DNA was originally unmethylated, a product will be generated
after PCR using the U primer set. Conversely, a product will be generated using
the M primer set if the sample was originally methylated.
相關(guān)PDF資料 |
PDF描述 |
|---|---|
| S7B-EH | BZ Series Standard Basic Switch, Single Pole Double Throw Circuitry, 15 A at 250 Vac, Overtravel Plunger Actuator, 2,5 N - 3,61 N [9 oz - 13 oz] Operating Force], Silver Contacts, Screw Termination, CE, CSA |
| S8040N | SCRs (1 A to 70 A) |
| S8040N | SCRs |
| S8040R | SCRs |
| S8055M | SCRs |
相關(guān)代理商/技術(shù)參數(shù) |
參數(shù)描述 |
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| S78-0400152-N40 | 制造商:Micro-Star International 功能描述:MSI CARD READER & WRITER - Bulk |
| S7805D | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805D_06 | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805P | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805PI | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
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