參數(shù)資料
型號: S7804
廠商: Electronic Theatre Controls, Inc.
英文描述: CpG WIZ Prader-Willi/Angelman Amplification Kit
中文描述: 中央人民政府武進Prader-Willi/Angelman擴增試劑盒
文件頁數(shù): 18/32頁
文件大?。?/td> 386K
代理商: S7804
14
Figure 3: Specificity of the CpG WIZ Prader-Willi/Angleman Amplification
Kit.
M 1 2 3 4
Polyacrylamide gel analysis using the primers and the control DNA samples
included in the kit is shown. (Lane 1: P primer set with modified control DNA;
Lane 2: M primer set with modified control DNA; Lane 3: P primer set with
unmodified control DNA; Lane 4: M primer set with unmodified control DNA.
Marker Lane: 100 bp ladder)
Experiment Setup
The CpG WIZ Prader-Willi/Angelman Amplification Kit includes sufficient
reagents to analyze 25 samples with appropriate controls (70 PCR reactions).
For each batch of experimental DNA samples to be analyzed, the experimental
samples and the normal control DNA must first undergo bisulfite modification.
In the subsequent PCR reactions, the chemically modified DNA samples, both
experimental and control, are amplified using both the maternal (M) and
paternal (P) primer sets. In addition, a negative PCR control (i.e. no DNA) is
performed for each set of primers.
For example, a typical gel for the analysis of five experimental DNA samples
and proper controls includes a total of 14 lanes:
Lanes 1-2
Experimental sample 1 with P and M primers
Lanes 3-4
Experimental sample 2 with P and M primers
Lanes 5-6
Experimental sample 3 with P and M primers
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