
i
_________________________________________________________
T
ABLE OF
C
ONTENTS
I. I
NTRODUCTION
..............................................................................1
Using this Manual..................................................................................... 1
Background............................................................................................... 1
Principles of the Technique....................................................................... 2
Fig 1: DNA Treatment with Sodium Bisulfite
....................................... 3
II. K
IT
C
OMPONENTS
.........................................................................4
Materials Required But Not Supplied....................................................... 5
III. P
ROTOCOLS
....................................................................................7
CpG WIZ p16 (S7800), p15 (S7802) and
E-cadherin (S7804) Amplification Kits ................................................. 7
Experimental Design............................................................................ 7
Fig. 2: Specificity of the CpG WIZ p16
Amplification Kit
.................................................................................. 9
Amplification Protocol....................................................................... 10
CpG WIZ Prader-Willi/Angelman (S7806) Amplification Kit........... 12
Experimental Design.......................................................................... 12
Fig. 3: Specificity of the CpG WIZ Prader-Willi/
Angelman Amplification Kit
............................................................... 14
Amplification Protocol....................................................................... 15
IV. D
ATA
A
NALYSIS
...........................................................................18
CpG WIZ p16 (S7800), p15 (S7802) and
E-cadherin (S7804) Amplification Kits ............................................. 18
CpG WIZ Prader-Willi/Angelman (S7806) Amplification Kit........... 19
V. T
ROUBLESHOOTING
....................................................................20
CpG WIZ p16 (S7800), p15 (S7802) and
E-cadherin (S7804) Amplification Kits .................................................. 20
CpG WIZ Prader-Willi/Angelman (S7806) Amplification Kit........... 21