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參數(shù)資料
| 型號： | S7804 |
| 廠商： | Electronic Theatre Controls, Inc. |
| 英文描述： | CpG WIZ Prader-Willi/Angelman Amplification Kit |
| 中文描述： | 中央人民政府武進Prader-Willi/Angelman擴增試劑盒 |
| 文件頁數(shù)： | 16/32頁 |
| 文件大?。?/td> | 386K |
| 代理商： | S7804 |
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f. Remove the tubes from the thermocycler block. From this point on, it is
important to designate separate pipettes and work areas for amplified vs.
unamplified samples. This prevents carry-over contamination of future DNA
samples with the amplified product.
STEP 3. Gel Electrophoresis
a. After the completion of PCR, add an appropriate amount of loading dye to
the sample and analyze 5 μL of the reaction on a 2% agarose, or a 10%
native acrylamide or other high resolution agarose gel. Use DNA markers
(100-300 bp range) to determine the size of PCR products.
b. After electrophoresis, stain the gel with ethidium bromide. Dilute the 10
mg/mL stock solution 1:10,000 in deionized water. Stain for 10-30 minutes
and destain for 10-30 minutes in deionized water at room temperature.
Note:
Ethidium bromide is a known carcinogen. Exercise appropriate
caution and good lab practice when using this reagent.
CpG WIZ Prader-Willi/Angelman (S7806) Amplification Kit
Experimental
Design
Primer Sets
The CpG WIZ Prader-Willi/Angelman Amplification Kit contains primers
that can be used for analysis of DNA samples by MSP. However, the first step
of MSP is the bisulfite modification of the DNA samples. CpGenome DNA
Modification Kit (S7820), contains the reagents necessary to perform the
modification. This chemical modification creates the sequence differences
between the methylated and unmethylated DNA. The primer sets in the kit are
engineered to anneal to the DNA, based upon the sequence differences.
P (Paternal) Primer Set will anneal to unmethylated DNA that has undergone a
chemical modification.
M (Maternal) Primer Set will anneal to methylated DNA that has undergone a
chemical modification.
相關(guān)PDF資料 |
PDF描述 |
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| S7B-EH | BZ Series Standard Basic Switch, Single Pole Double Throw Circuitry, 15 A at 250 Vac, Overtravel Plunger Actuator, 2,5 N - 3,61 N [9 oz - 13 oz] Operating Force], Silver Contacts, Screw Termination, CE, CSA |
| S8040N | SCRs (1 A to 70 A) |
| S8040N | SCRs |
| S8040R | SCRs |
| S8055M | SCRs |
相關(guān)代理商/技術(shù)參數(shù) |
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| S78-0400152-N40 | 制造商:Micro-Star International 功能描述:MSI CARD READER & WRITER - Bulk |
| S7805D | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805D_06 | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805P | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805PI | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
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