- 您現(xiàn)在的位置：買賣IC網(wǎng) > PDF目錄374671 > S7804 (Electronic Theatre Controls, Inc.) CpG WIZ Prader-Willi/Angelman Amplification Kit PDF資料下載
參數(shù)資料
| 型號(hào)： | S7804 |
| 廠商： | Electronic Theatre Controls, Inc. |
| 英文描述： | CpG WIZ Prader-Willi/Angelman Amplification Kit |
| 中文描述： | 中央人民政府武進(jìn)Prader-Willi/Angelman擴(kuò)增試劑盒 |
| 文件頁(yè)數(shù)： | 13/32頁(yè) |
| 文件大小： | 386K |
| 代理商： | S7804 |
第1頁(yè)第2頁(yè)第3頁(yè)第4頁(yè)第5頁(yè)第6頁(yè)第7頁(yè)第8頁(yè)第9頁(yè)第10頁(yè)第11頁(yè)第12頁(yè)當(dāng)前第13頁(yè)第14頁(yè)第15頁(yè)第16頁(yè)第17頁(yè)第18頁(yè)第19頁(yè)第20頁(yè)第21頁(yè)第22頁(yè)第23頁(yè)第24頁(yè)第25頁(yè)第26頁(yè)第27頁(yè)第28頁(yè)第29頁(yè)第30頁(yè)第31頁(yè)第32頁(yè)
9
Specificity of the Assay
The specificity of the CpG WIZ p16 Amplification Kit is shown in Figure 2.
With a complete chemical modification reaction, U primers amplify only
unmethylated DNA (154 bp, lane 1) and M primers amplify only methylated
DNA (145 bp, lane 5). W primers amplify only DNA which is not chemically
modified, or "wild type W" (142 bp, lane 9).
Figure 2: Specificity of the CpG WIZ p16 Amplification Kit.
M 1 2 3 4 5 6 7 8 9 10 11 12 M
Polyacrylamide gel analysis using the primers and the control DNA samples
included in the kit is shown. (Lanes 1-3: U control DNA; lanes 4-6: M control
DNA; lanes 7-9: W control DNA; lanes 10-12: Minus DNA control. Lanes 1, 4,
7, 10: U primers; lanes 2, 5, 8, 11: M primers; lanes 3, 6, 9, 12: W primers. M
lane: 100 base-pair marker.)
Experiment Setup
CpG WIZ Amplification Kits (S7800, S7802, and S7804) include sufficient
reagents to analyze 25 samples with appropriate controls (105 PCR reactions).
Each experiment will include chemical modification of seven DNAs: 5
experimental DNA and 2 control DNA samples, (M and U). In the subsequent
PCR reactions, each chemically modified experimental DNA sample is
amplified with each of three oligonucleotide primer sets U, M and W. The
chemically modified genomic control DNAs, U and M, are amplified with their
corresponding primer set. Untreated W genomic control DNA is amplified with
the W primer set. Lastly, a negative PCR control (i.e. no DNA) is performed for
each set of primers.
相關(guān)PDF資料 |
PDF描述 |
|---|---|
| S7B-EH | BZ Series Standard Basic Switch, Single Pole Double Throw Circuitry, 15 A at 250 Vac, Overtravel Plunger Actuator, 2,5 N - 3,61 N [9 oz - 13 oz] Operating Force], Silver Contacts, Screw Termination, CE, CSA |
| S8040N | SCRs (1 A to 70 A) |
| S8040N | SCRs |
| S8040R | SCRs |
| S8055M | SCRs |
相關(guān)代理商/技術(shù)參數(shù) |
參數(shù)描述 |
|---|---|
| S78-0400152-N40 | 制造商:Micro-Star International 功能描述:MSI CARD READER & WRITER - Bulk |
| S7805D | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805D_06 | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805P | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805PI | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
發(fā)布緊急采購(gòu)，3分鐘左右您將得到回復(fù)。