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參數(shù)資料
| 型號(hào)： | S7802 |
| 廠商： | Electronic Theatre Controls, Inc. |
| 英文描述： | CpG WIZ Prader-Willi/Angelman Amplification Kit |
| 中文描述： | 中央人民政府武進(jìn)Prader-Willi/Angelman擴(kuò)增試劑盒 |
| 文件頁數(shù)： | 11/32頁 |
| 文件大小： | 386K |
| 代理商： | S7802 |
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7
III.
P
ROTOCOLS
CpG WIZ p16 (S7800), p15 (S7802) and E-cadherin (S7804)
Amplification Kits
Experimental Design
Primer Sets
CpG WIZ Amplification Kits contain primers that can be used for analysis of
DNA samples by MSP. However, the samples must first undergo bisulfite
modification prior to PCR amplification. CpGenome DNA Modification Kit,
S7820, contains the reagents necessary to perform the modification. Chemical
modification creates the sequence differences between the methylated and
unmethylated DNA. The primer sets in the kit are engineered to anneal to the
DNA, based upon the sequence differences.
U Primer Set will anneal to unmethylated DNA that has undergone a chemical
modification
M Primer Set will anneal to methylated DNA that has undergone a chemical
modification
W Primer Set serves as a control for the efficiency of chemical modification. It
will anneal to
any
DNA (unmethylated or methylated) that has NOT undergone
chemical modification, hence, the "wild type", or W.
Data interpretation can still proceed in the case of incomplete chemical
modification (up to 50%).
Amplification Regions
The amplified region is defined as the sequence between the 3' nucleotide of the
sense primer and the complement of the 3' nucleotide of the anti-sense primer
for each gene promoter. The nucleotide numbering systems are those used in the
GenBank submissions identified by the following accession numbers:
p16
,
X94154;
p15
, S75756;
E-cadherin
, L34545.
相關(guān)PDF資料 |
PDF描述 |
|---|---|
| S7804 | CpG WIZ Prader-Willi/Angelman Amplification Kit |
| S7B-EH | BZ Series Standard Basic Switch, Single Pole Double Throw Circuitry, 15 A at 250 Vac, Overtravel Plunger Actuator, 2,5 N - 3,61 N [9 oz - 13 oz] Operating Force], Silver Contacts, Screw Termination, CE, CSA |
| S8040N | SCRs (1 A to 70 A) |
| S8040N | SCRs |
| S8040R | SCRs |
相關(guān)代理商/技術(shù)參數(shù) |
參數(shù)描述 |
|---|---|
| S7804 | 制造商:未知廠家 制造商全稱:未知廠家 功能描述:CpG WIZ Prader-Willi/Angelman Amplification Kit |
| S78-0400152-N40 | 制造商:Micro-Star International 功能描述:MSI CARD READER & WRITER - Bulk |
| S7805D | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805D_06 | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
| S7805P | 制造商:AUK 制造商全稱:AUK corp 功能描述:Fixed Voltage Regulator |
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