參數(shù)資料
型號(hào)： S7800
廠商： Electronic Theatre Controls, Inc.
英文描述： CpG WIZ Prader-Willi/Angelman Amplification Kit
中文描述： 中央人民政府武進(jìn)Prader-Willi/Angelman擴(kuò)增試劑盒
文件頁數(shù)： 27/32頁
文件大?。?/td> 386K
代理商： S7800
23
VI.
A
PPENDIX
Laboratory Setup and Precautions
One of the most important considerations when performing MSP using CpG
WIZ Amplification Kits is the environment where the initial reaction mixtures
are set up. The ideal environment is free of amplified DNA products, which can
cause false-positive results. Potential sources of PCR product contamination are:
contaminated pipettors and tips, gel box and buffer, tube racks, notebooks, lab
coats and any other item exposed to amplified PCR products.
The following precautions should be followed in all steps of the assay protocol:
a. Always wear gloves.
b. Use sterile water for all solutions, aliquot the solutions in small amounts, and
use fresh aliquots as working solutions. Discard working solutions after use.
c. Keep the assay solutions (10X PCR buffers, dNTPs, polymerase, etc.)
separate from the amplified DNA.
d. Always use aerosol resistant pipette tips.
e. Separate micropipettors and work areas are recommended for the following
three steps of the assay:
1. DNA modification and purification
2. Amplification setup
3. Post-amplification analysis
Related Products
Product
Catalog Number
CpGenome DNA Modification Kit
S7820
CpGenome Universal Methylated DNA (human
male genomic DNA)
S7821
DNA Extraction Kit, Non-Organic
S4520
EX-WAX DNA Extraction Kit
for paraffin-embedded tissue
S4530
相關(guān)PDF資料
PDF描述
S7802 CpG WIZ Prader-Willi/Angelman Amplification Kit
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S7804 制造商:未知廠家 制造商全稱:未知廠家 功能描述:CpG WIZ Prader-Willi/Angelman Amplification Kit
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